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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CABLES1 Polyclonal antibody (13464-1-AP)

    WB analysis using 13464-1-AP (same clone as 13464-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13464-1-AP (CABLES1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13464-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using CABLES1 Polyclonal antibody (13464-1-AP)

    IHC staining of human colon using 13464-1-AP (same clone as 13464-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13464-1-AP (CABLES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13464-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using CABLES1 Polyclonal antibody (13464-1-AP)

    IHC staining of mouse pancreas using 13464-1-AP (same clone as 13464-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13464-1-AP (CABLES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13464-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CABLES1 Polyclonal antibody (13464-1-AP)

    IF Staining of MCF-7 using 13464-1-AP (same clone as 13464-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CABLES1 antibody (13464-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13464-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CABLES1 Polyclonal antibody (13464-1-AP)

    IF Staining of HeLa using 13464-1-AP (same clone as 13464-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CABLES1 antibody (13464-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13464-1-PBS in a different storage buffer formulation.

CABLES1 Polyclonal antibody, PBS Only

CABLES1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13464-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Ik3-1, HsT2563, CABLES

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13464-1-PBS targets CABLES1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4251

Product name: Recombinant human CABLES1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-368 aa of BC037218

Sequence: SQRSSLETLEDIEENAPLRRCRTLSGSPRPKNFKKIHFIKNMRQHDTRNGRIVLISGRRSFCSIFSVLPYRDSTQVGDLKLDGGRQSTGAVSLKEIIGLEGVELGADGKTVSYTQFLLPTNAFGARRNTIDSTSSFSQFRNLSHRSLSIGRASGTQGSLDTGSDLGDFMDYDPNLLDDPQWPCGKHKRVLIFPSYMTTVIDYVKPSDLKKDMNETFKEKFPHIKLTLSKIRSLKREMRKLAQEDCGLEEPTVAMAFVYFEKLALKGKLNKQNRKLCAGACVLLAAKIGSDLKKHEVKHLIDKLEEKFRLNRRELIAFEFPVLVALEFALHLPEHEVMPHYRRLVQSS

相同性解析による交差性が予測される生物種
Full Name Cdk5 and Abl enzyme substrate 1
Calculated molecular weight 568 aa, 63 kDa
Observed molecular weight68 kDa, 42 kDa
GenBank accession numberBC037218
Gene Symbol CABLES1
Gene ID (NCBI) 91768
RRIDAB_3669163
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDN4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CDK5 and ABL1 enzyme substrate 1 (CABLES1) is a novel Cdk2, Cdk3, and Cdk5 binding protein, which acts as a link between the Cdks and nonreceptor tyrosine kinases and regulates the activity of Cdks by enhancing their Y15 phosphorylation. CABLES1 is a candidate tumor suppressor that negatively regulates cell growth by inhibiting cyclin-dependent kinases.

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