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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using CAB39L Polyclonal antibody (16137-1-AP)

    WB analysis of K-562 using 16137-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16137-1-AP (CAB39L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CAB39L Polyclonal antibody (16137-1-AP)

    WB analysis of HEK-293 using 16137-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16137-1-AP (CAB39L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using CAB39L Polyclonal antibody (16137-1-AP)

    WB analysis of human spleen using 16137-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 16137-1-AP (CAB39L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CAB39L Polyclonal antibody (16137-1-AP)

    IHC staining of human lymphoma using 16137-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16137-1-AP (CAB39L Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CAB39L Polyclonal antibody (16137-1-AP)

    IHC staining of human lymphoma using 16137-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16137-1-AP (CAB39L Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CAB39L Polyclonal antibody (16137-1-AP)

    IF Staining of HEK-293 using 16137-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CAB39L antibody (16137-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CAB39L Polyclonal antibody

CAB39L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16137-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Mo25-like protein, Mo25 like protein, MO25 BETA, Calcium-binding protein 39-like, Antigen MLAA-34

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, human spleen tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

16137-1-AP targets CAB39L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9181

Product name: Recombinant human CAB39L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-337 aa of BC010993

Sequence: MKKMPLFSKSHKNPAEIVKILKDNLAILEKQDKKTDKASEEVSKSLQAMKEILCGTNEKEPPTEAVAQLAQELYSSGLLVTLIADLQLIDFEEKKDVTQIFNNILRRQIGTRSPTVEYISAHPHILFMLLKGYEAPQIALRCGIMLRECIRHEPLAKIILFSNQFRDFFKYVELSTFDIASDAFATFKDLLTRHKVLVADFLEQNYDTIFEDYEKLLQSENYVTKRQSLKLLGELILDRHNFAIMTKYISKPENLKLMMNLLRDKSPNIQFEAFHVFKVFVASPHKTQPIVEILLKNQPKLIEFLSSFQKERTDDEQFADEKNYLIKQIRDLKKTAP

相同性解析による交差性が予測される生物種
Full Name calcium binding protein 39-like
Calculated molecular weight 337 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC010993
Gene Symbol CAB39L
Gene ID (NCBI) 81617
RRIDAB_2066394
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9S4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CAB39L antibody 16137-1-APDownload protocol
IHC protocol for CAB39L antibody 16137-1-APDownload protocol
WB protocol for CAB39L antibody 16137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Medicina (Kaunas)

Bioinformatics Prediction and Experimental Verification Identify CAB39L as a Diagnostic and Prognostic Biomarker of Kidney Renal Clear Cell Carcinoma

Authors - Yunfei Wu
View Article
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