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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CA7 Polyclonal antibody (13670-1-AP)

    WB analysis using 13670-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CA7 Polyclonal antibody (13670-1-AP)

    WB analysis using 13670-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human liver cancer using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human liver cancer using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human stomach using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human stomach using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human kidney using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human kidney using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CA7 Polyclonal antibody (13670-1-AP)

    IF Staining of HeLa using 13670-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13670-1-AP (CA7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CA7 Polyclonal antibody

CA7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13670-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CA-VII, CAVII, carbonic anhydrase VII, Carbonic anhydrase 7, Carbonate dehydratase VII

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13670-1-AP targets CA7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4649

Product name: Recombinant human CA7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-264 aa of BC033865

Sequence: MTGHHGWGYGQDDGPSHWHKLYPIAQGDRQSPINIISSQAVYSPSLQPLELSYEACMSLSITNNGHSVQVDFNDSDDRTVVTGGPLEGPYRLKQFHFHWGKKHDVGSEHTVDGKSFPSELHLVHWNAKKYSTFGEAASAPDGLAVVGVFLETGDEHPSMNRLTDALYMVRFKGTKAQFSCFNPKCLLPASRHYWTYPGSLTTPPLSESVTWIVLREPICISERQMGKFRSLLFTSEDDERIHMVNNFRPPQPLKGRVVKASFRA

相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase VII
Calculated molecular weight 264 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC033865
Gene Symbol CA7
Gene ID (NCBI) 766
RRIDAB_2243629
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP43166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CA7 antibody 13670-1-APDownload protocol
IHC protocol for CA7 antibody 13670-1-APDownload protocol
WB protocol for CA7 antibody 13670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse,rat,pigIF

Cell Regen

Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium.

Authors - Haonan Li
View Article
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