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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CA4 Recombinant antibody (85706-1-RR)

    WB analysis using 85706-1-RR (same clone as 85706-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85706-1-RR (CA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85706-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CA4 Recombinant antibody (85706-1-RR)

    IHC staining of mouse lung using 85706-1-RR (same clone as 85706-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85706-1-RR (CA4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85706-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85706-1-RR

    Affinity and Kinetic Characterization of 85706-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85706-1-RR against Human CA4 were performed. The affinity constant is 9.62 nM.

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CA4 Recombinant antibody, PBS Only

CA4 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85706-1-PBS
Clone No.242975A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

CA IV, CAIV, CA-IV, Car4, Carbonate dehydratase IV

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85706-1-PBS targets CA4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CA4 fusion protein Ag4960 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase IV
Calculated molecular weight 35 kDa
Observed molecular weight35 kDa
GenBank accession numberBC057792
Gene Symbol CA4
Gene ID (NCBI) 762
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22748
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CA4(Carbonic anhydrase 4) is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary, chorionic (and certain other) capillaries and of proximal renal tubules. It plays a critical role by maintaining the pH in the outer retina, which is important for the normal function of photoreceptors. This protein belongs to the alpha-carbonic anhydrase family.