Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, RAW 264.7 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86000-1-RR targets CA1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | CA1 fusion protein Ag3932 相同性解析による交差性が予測される生物種 |
Full Name | carbonic anhydrase I |
Calculated molecular weight | 261 aa, 29 kDa |
Observed molecular weight | 29 kDa |
GenBank accession number | BC027890 |
Gene Symbol | CA1 |
Gene ID (NCBI) | 759 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P00915 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CA1(Carbonic anhydrase 1) is also named as CAB and belongs to the alpha-carbonic anhydrase family, which may reside in cytoplasm, in mitochondria, or in secretory granules, or associate with membranes in cell. It forms a large family of genes encoding zinc metalloenzymes of great physiologic importance. Extracellular CA1 mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation(PMID:17259996).
Protocols
Product Specific Protocols | |
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WB protocol for CA1 antibody 86000-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |