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Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using C9orf86 Polyclonal antibody (20848-1-AP)

    WB analysis of MDA-MB-453s using 20848-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20848-1-AP (C9orf86 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C9orf86 Polyclonal antibody (20848-1-AP)

    IHC staining of human breast cancer using 20848-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20848-1-AP (C9orf86 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C9orf86 Polyclonal antibody (20848-1-AP)

    IHC staining of human breast cancer using 20848-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20848-1-AP (C9orf86 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C9orf86 Polyclonal antibody (20848-1-AP)

    IF Staining of HeLa using 20848-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20848-1-AP (C9orf86 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C9orf86 Polyclonal antibody (20848-1-AP)

    IF Staining of HeLa using 20848-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C9orf86 antibody (20848-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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C9orf86 Polyclonal antibody

C9orf86 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20848-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RBEL1, Rab-like protein 6, Rab-like protein 1, pp8875, Partner of ARF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20848-1-AP targets C9orf86 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf86 fusion protein Ag14350 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 86
Calculated molecular weight 729 aa, 80 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC002945
Gene Symbol C9orf86
Gene ID (NCBI) 55684
RRIDAB_2878750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3YEC7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C9orf86, also known as RBEL1 (Rab-like protein 1), is a novel subfamily of GTPases within the Ras superfamily. It has two splice variants, RBEL1Aand RBEL1B. Unlike known Rabs that aremostly cytosolic, RBEL1B predominantly resides in the nucleus, whereas RBEL1A is localized primarily to the cytosol. C9orf86 is overexpressed in the majority of primary breast tumors, and knockdown of C9orf86 in MCF-7 breast cancer cells resulted in cell growth suppression associated with apoptosis [PMID: 23977139]. Western blot analysis revealed that the endogenous RBEL1 migrated at 110-125 kDa, 80 kDa, maybe due to a number of potential O-linked glycosylation sites.[PMID: 17962191]

Protocols

Product Specific Protocols
WB protocol for C9orf86 antibody 20848-1-APDownload protocol
IHC protocol for C9orf86 antibody 20848-1-APDownload protocol
IF protocol for C9orf86 antibody 20848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

m5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization

Authors - Ning Wang
View Article