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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C9orf7 / CACFD1 Polyclonal antibody (21330-1-AP)

    WB analysis using 21330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21330-1-AP (C9orf7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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C9orf7 / CACFD1 Polyclonal antibody

C9orf7 / CACFD1 Polyclonal Antibody for WB, ELISA
Cat No. 21330-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

C9orf7, CACFD1, Calcium channel flower homolog, D9S2135, FWE, hFWE, PSEC0248, UNQ3071/PRO9903

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21330-1-AP targets C9orf7 / CACFD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf7 / CACFD1 fusion protein Ag15336 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 7
Calculated molecular weight 172 aa, 18 kDa
Observed molecular weight~25 kDa
GenBank accession numberBC030558
Gene Symbol CACFD1
Gene ID (NCBI) 11094
RRIDAB_3085651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGQ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcium Channel Flower Domain Containing 1 (CACFD1) also named as C9orf7 or human Flower protein (hFWE). In Drosophila, cell selection uses a cell selection mechanism based on 'fitness fingerprints', which allow it to delay ageing, prevent developmental malformations and replace old tissues during regeneration. At the molecular level, these fitness fingerprints consist of combinations of Flower membrane proteins. Proteins that indicate reduced fitness are called Flower-Lose, because they are expressed in cells marked to be eliminated. It has different isoforms, the calculated MW rang from 14-25 kDa. 21330-1-AP can detect a band around 25 kDa.(PMID:31341286)

Protocols

Product Specific Protocols
WB protocol for C9orf7 / CACFD1 antibody 21330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols