Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
25630-1-AP targets C9orf61 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22242 Product name: Recombinant human C9orf61 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 288-450 aa of BC113685 Sequence: PVLSCEAATQTERRLDLAAVTLRRGLRSRASRCRPRSLIDYKSYMDTKLLVARFLEQSSCTMTPDIHELVENIKSVLKSDEEHMEEAITSASFLEQIMAPLQPSTSRAHKLPSRRQPGLLHLQSCGDLHTFTPAGRPRAERRPRRVEAERPHSLIGVIRETVL 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 9 open reading frame 61 |
| Calculated molecular weight | 450 aa, 50 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC113685 |
| Gene Symbol | C9orf61 |
| Gene ID (NCBI) | 9413 |
| RRID | AB_2880170 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15884 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C9orf61, also named as FAM189A2 and X123, is promoinently expressed in muscle. It has three isoforms with MW 45-50 kDa, 32-35 kDa and 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C9orf61 antibody 25630-1-AP | Download protocol |
| IHC protocol for C9orf61 antibody 25630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |