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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C9orf156 Polyclonal antibody

C9orf156 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20475-1-AP

Synonyms

C9orf156, HSPC219, NAP1, Nef associated protein 1, Thioesterase NAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using C9orf156 Polyclonal antibody (20475-1-AP)

    WB analysis of HeLa using 20475-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20475-1-AP (C9orf156 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using C9orf156 Polyclonal antibody (20475-1-AP)

    WB analysis of Jurkat using 20475-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20475-1-AP (C9orf156 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using C9orf156 Polyclonal antibody (20475-1-AP)

    WB analysis of HepG2 using 20475-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20475-1-AP (C9orf156 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using C9orf156 Polyclonal antibody (20475-1-AP)

    IHC staining of human lung cancer using 20475-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 20475-1-AP (C9orf156 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using C9orf156 Polyclonal antibody (20475-1-AP)

    IHC staining of human lung cancer using 20475-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 20475-1-AP (C9orf156 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20475-1-AP is C9orf156 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf156 fusion protein Ag14335 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 156
Calculated molecular weight 441 aa, 49 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC002863
Gene symbol C9orf156
Gene ID (NCBI) 51531
RRIDAB_10665553
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C9orf156 antibody 20475-1-APDownload protocol
IHC protocol for C9orf156 antibody 20475-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols