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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using C9 Recombinant monoclonal antibody (84890-4-RR)

    WB analysis of human placenta using 84890-4-RR (same clone as 84890-4-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 84890-4-RR (C9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84890-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using C9 Recombinant monoclonal antibody (84890-4-RR)

    IHC staining of human colon using 84890-4-RR (same clone as 84890-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84890-4-RR (C9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84890-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using C9 Recombinant monoclonal antibody (84890-4-RR)

    IHC staining of human tonsillitis using 84890-4-RR (same clone as 84890-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84890-4-RR (C9 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84890-4-PBS in a different storage buffer formulation.

  • Affinity of 84890-4-RR

    Affinity of 84890-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84890-4-RR against Human C9 were performed. The affinity constant is 0.739 nM.

C9 Recombinant monoclonal antibody, PBS Only

C9 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84890-4-PBS
Clone No.242475B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Complement C9, complement component 9, Complement component C9, Complement component C9a, Complement component C9b

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84890-4-PBS targets C9 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg1282

Product name: Recombinant Human Component C9 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 22-559 aa of NM_001737.4

Sequence: QYTTSYDPELTESSGSASHIDCRMSPWSEWSQCDPCLRQMFRSRSIEVFGQFNGKRCTDAVGDRRQCVPTEPCEDAEDDCGNDFQCSTGRCIKMRLRCNGDNDCGDFSDEDDCESEPRPPCRDRVVEESELARTAGYGINILGMDPLSTPFDNEFYNGLCNRDRDGNTLTYYRRPWNVASLIYETKGEKNFRTEHYEEQIEAFKSIIQEKTSNFNAAISLKFTPTETNKAEQCCEETASSISLHGKGSFRFSYSKNETYQLFLSYSSKKEKMFLHVKGEIHLGRFVMRNRDVVLTTTFVDDIKALPTTYEKGEYFAFLETYGTHYSSSGSLGGLYELIYVLDKASMKRKGVELKDIKRCLGYHLDVSLAFSEISVGAEFNKDDCVKRGEGRAVNITSENLIDDVVSLIRGGTRKYAFELKEKLLRGTVIDVTDFVNWASSINDAPVLISQKLSPIYNLVPVKMKNAHLKKQNLERAIEDYINEFSVRKCHTCQNGGTVILMDGKCLCACPFKFEGIACEISKQKISEGLPALEFPNEK

相同性解析による交差性が予測される生物種
Full Name complement component 9
Calculated molecular weight63kDa
Observed molecular weight64 kDa
GenBank accession numberNM_001737.4
Gene Symbol C9
Gene ID (NCBI) 735
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP02748
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The final consequence of complement activation is the formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. The MAC plays a critical role in complement-mediated hemolysis and inflammasome activation (PMID: 27444648). Complement component 9 (C9) is 1 of 5 late-acting proteins of complement that participate in the assembly and function of the MAC. C9 may be cleaved by thrombin to generate fragments C9a (25 kDa) and C9b (37 kDa) (PMID: 7523406).

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