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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C8orf4 Polyclonal antibody

C8orf4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26279-1-AP

Synonyms

C8orf4, hTC 1, TC 1, TC1, Thyroid cancer protein 1, Uncharacterized protein C8orf4

Validation Data Gallery

Applications:
  • WB analysis of mouse kidney using 26279-1-AP

    WB analysis of mouse kidney using 26279-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26279-1-AP (C8orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 26279-1-AP

    WB analysis of HEK-293 using 26279-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26279-1-AP (C8orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 26279-1-AP

    IHC staining of human thyroid cancer using 26279-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26279-1-AP (C8orf4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human thyroid cancer using 26279-1-AP

    IHC staining of human thyroid cancer using 26279-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26279-1-AP (C8orf4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 26279-1-AP

    IHC staining of human kidney using 26279-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26279-1-AP (C8orf4 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 26279-1-AP

    IHC staining of human kidney using 26279-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26279-1-AP (C8orf4 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells
Positive IHC detected inhuman thyroid cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 26279-1-AP is C8orf4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C8orf4 fusion protein Ag23930 相同性解析による交差性が予測される生物種
Full Name chromosome 8 open reading frame 4
Observed molecular weight 14 kDa
GenBank accession numberBC021672
Gene symbol C8orf4
Gene ID (NCBI) 56892
RRIDAB_2880460
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C8orf4 antibody 26279-1-APDownload protocol
IHC protocol for C8orf4 antibody 26279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Medicine (Baltimore)

Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous cell carcinoma

Authors - Gang Ren
View Article