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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C8G Recombinant antibody (85652-1-RR)

    WB analysis using 85652-1-RR (same clone as 85652-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85652-1-RR (C8G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85652-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using C8G Recombinant antibody (85652-1-RR)

    WB analysis of human plasma using 85652-1-RR (same clone as 85652-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 85652-1-RR (C8G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85652-1-PBS in a different storage buffer formulation.

C8G Recombinant monoclonal antibody, PBS Only

C8G Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85652-1-PBS
Clone No.242479G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

C8C

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85652-1-PBS targets C8G in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag18261

Product name: Recombinant human C8G protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-202 aa of BC113626

Sequence: MLPPGTATLLTLLLAAGSLGQKPQRPRRPASPISTIQPKANFDAQQFAGTWLLVAVGSACRFLQEQGHRAEATTLHVAPQGTAMAVSTFRKLDGICWQVRQLYGDTGVLGRFLLQARGARGAVNVVVAETDYQSFAVLYLERAGQLSVKLYARSLPVSDSVLSGFEQRVQEAHLTEDQIFYFPKYGFCEAADQFHVLDEVRR

相同性解析による交差性が予測される生物種
Full Name complement component 8, gamma polypeptide
Calculated molecular weight 202 aa, 22 kDa
Observed molecular weight18-22 kDa
GenBank accession numberBC113626
Gene Symbol C8G
Gene ID (NCBI) 733
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP07360
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The gamma chain of complement component 8 (C8G) is one of three different subunits (alpha, beta, and gamma) of the eighth complement component (C8), which is a component of the MAC (membrane attack complex) (PMID:33382892). Although the roles of C8A and C8B are directly associated with MAC formation, C8G is not essential in the assembly and activity of the MAC (PMID:11960635). Moreover, C8G was shown to inhibit neuroinflammation by interfering with the sphingosine-1-phosphate (S1P)-S1PR2 interaction (PMID:34149451).

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