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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C8G Polyclonal antibody (27200-1-AP)

    WB analysis using 27200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27200-1-AP (C8G antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using C8G Polyclonal antibody (27200-1-AP)

    WB analysis of human placenta using 27200-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27200-1-AP (C8G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C8G Polyclonal antibody (27200-1-AP)

    IHC staining of human breast cancer using 27200-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C8G Polyclonal antibody (27200-1-AP)

    IHC staining of human liver cancer using 27200-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C8G Polyclonal antibody (27200-1-AP)

    IHC staining of human breast cancer using 27200-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C8G Polyclonal antibody (27200-1-AP)

    IHC staining of human liver cancer using 27200-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C8G Polyclonal antibody

C8G Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27200-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C8C

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman milk, human placenta tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27200-1-AP targets C8G in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17949

Product name: Recombinant human C8G protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC113626

Sequence: MLPPGTATLLTLLLAAGSLGQKPQRPRRPASPISTIQPKANFDAQQFAGTWLLVAVGSACRFLQEQGHRAEATTLHVAPQGTAMAVSTFRKLDGICWQVRQLYGDTGVLGRFLLQARGARGAVNVVVAETDYQSFAVLYLERAGQLSVKLYARSLPVSDSVLSGFEQRVQEAHLTEDQIFYFPKYGFCEAADQFHVLDEVRR

相同性解析による交差性が予測される生物種
Full Name complement component 8, gamma polypeptide
Calculated molecular weight 202 aa, 22 kDa
Observed molecular weight18-22 kDa
GenBank accession numberBC113626
Gene Symbol C8G
Gene ID (NCBI) 733
RRIDAB_3085935
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07360
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The gamma chain of complement component 8 (C8G) is one of three different subunits (alpha, beta, and gamma) of the eighth complement component (C8), which is a component of the MAC (membrane attack complex) (PMID:33382892). Although the roles of C8A and C8B are directly associated with MAC formation, C8G is not essential in the assembly and activity of the MAC (PMID:11960635). Moreover, C8G was shown to inhibit neuroinflammation by interfering with the sphingosine-1-phosphate (S1P)-S1PR2 interaction (PMID:34149451).

Protocols

Product Specific Protocols
WB protocol for C8G antibody 27200-1-APDownload protocol
IHC protocol for C8G antibody 27200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols