Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25184-1-AP targets C8B in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18388 Product name: Recombinant human C8B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 242-591 aa of BC130575 Sequence: RNVTEKMASKSGFSFGFKIPGIFELGISSQSDRGKHYIRRTKRFSHTKSVFLHARSDLEVAHYKLKPRSLMLHYEFLQRVKRLPLEYSYGEYRDLFRDFGTHYITEAVLGGIYEYTLVMNKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLGVSVGKCRGILNEIKDRNKRDTMVEDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVKVEPLYELVTATDFAYSSTVRQNMKQALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYRKNTPIDGKWNCWSNWSSCSGRRKTRQRQCNNPPPQNGGSPCSGPASETLDCS 相同性解析による交差性が予測される生物種 |
| Full Name | complement component 8, beta polypeptide |
| Calculated molecular weight | 591 aa, 67 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC130575 |
| Gene Symbol | C8B |
| Gene ID (NCBI) | 732 |
| RRID | AB_3085774 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07358 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The beta chain of complement component 8 (C8B) is one of three different subunits (alpha, beta, and gamma) of the complement component 8 (C8), which is a component of the MAC (membrane attack complex). The deficiency of C8B leads to the type II of C8 deficiency, leading to the malfunction of MAC.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for C8B antibody 25184-1-AP | Download protocol |
| WB protocol for C8B antibody 25184-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |