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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C7orf58 Polyclonal antibody

C7orf58 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20924-1-AP

Synonyms

C7orf58, CPED1

Validation Data Gallery

Applications:
  • WB analysis of A375 using 20924-1-AP

    WB analysis of A375 using 20924-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 20924-1-AP

    WB analysis of L02 using 20924-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 20924-1-AP

    WB analysis of A431 using 20924-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 20924-1-AP

    WB analysis of HeLa using 20924-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 20924-1-AP

    WB analysis of HEK-293 using 20924-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20924-1-AP (C7orf58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 20924-1-AP

    IHC staining of human stomach using 20924-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20924-1-AP (C7orf58 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human stomach using 20924-1-AP

    IHC staining of human stomach using 20924-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20924-1-AP (C7orf58 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of A375 using 20924-1-AP

    IF Staining of A375 using 20924-1-AP

    Immunofluorescent analysis of A375 cells using 20924-1-AP (C7orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, A431 cells, HEK-293 cells, HeLa cells, L02 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20924-1-AP is C7orf58 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C7orf58 fusion protein Ag15193 相同性解析による交差性が予測される生物種
Full Name chromosome 7 open reading frame 58
Calculated molecular weight 1026 aa, 117 kDa
Observed molecular weight 89 kDa
GenBank accession numberBC030538
Gene symbol C7orf58
Gene ID (NCBI) 79974
RRIDAB_10697813
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C7orf58 antibody 20924-1-APDownload protocol
IHC protocol for C7orf58 antibody 20924-1-APDownload protocol
IF protocol for C7orf58 antibody 20924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

FN1 mRNA 3'-UTR supersedes traditional fibronectin 1 in facilitating the invasion and metastasis of gastric cancer through the FN1 3'-UTR-let-7i-5p-THBS1 axis

Authors - Siwei Pan
View Article