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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C7orf27 Polyclonal antibody (31921-1-AP)

    WB analysis using 31921-1-AP (same clone as 31921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31921-1-AP (C7orf27 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using C7orf27 Polyclonal antibody (31921-1-AP)

    IHC staining of human placenta using 31921-1-AP (same clone as 31921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31921-1-AP (C7orf27 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31921-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C7orf27 Polyclonal antibody (31921-1-AP)

    IF Staining of HEK-293 using 31921-1-AP (same clone as 31921-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C7orf27 antibody (31921-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31921-1-PBS in a different storage buffer formulation.

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C7orf27 Polyclonal antibody, PBS Only

C7orf27 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31921-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31921-1-PBS targets C7orf27 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C7orf27 fusion protein Ag37056 相同性解析による交差性が予測される生物種
Full Name chromosome 7 open reading frame 27
Observed molecular weight75 kDa
GenBank accession numberBC015632
Gene Symbol C7orf27
Gene ID (NCBI) 221927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ6PJG6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

C7orf27, also known as BRAT1, not only plays a role in DNA damage response, but also participates in the regulation of cell growth and apoptosis, which further regulates mitochondrial metabolism and tumorigenesis. Moreover, several studies have shown that BRAT1 mutations are associated with neurodevelopmental and neurodegenerative diseases.