Validation Data Gallery
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Tested Applications
| Positive WB detected in | PC-13 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:800 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
24588-1-AP targets CEP85L in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CEP85L fusion protein Ag20235 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 6 open reading frame 204 |
| Calculated molecular weight | 805 aa, 92 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC153054 |
| Gene Symbol | CEP85L |
| Gene ID (NCBI) | 387119 |
| RRID | AB_2819012 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5SZL2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CEP85L, also named as C60rf204, is a gene which encodes a protein that has identified as a breast cancer antigen. Nothing more is known of its function at this time. There're five transcript variants with MW 45 kDa, 55-56 kDa and 91-92 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP85L antibody 24588-1-AP | Download protocol |
| IHC protocol for CEP85L antibody 24588-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Pathogenic Variants in CEP85L Cause Sporadic and Familial Posterior Predominant Lissencephaly. | ||
Neuron Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.
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