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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C6orf130 Recombinant antibody (83500-4-RR)

    WB analysis using 83500-4-RR (same clone as 83500-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83500-4-RR (C6orf130 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83500-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using C6orf130 Recombinant antibody (83500-4-RR)

    FC experiment of A431 using 83500-4-RR (same clone as 83500-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug C6orf130 Recombinant antibody (83500-4-RR, Clone:240455D7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83500-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using C6orf130 Recombinant antibody (83500-4-RR)

    FC experiment of PC-3 using 83500-4-RR (same clone as 83500-4-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug C6orf130 Recombinant antibody (83500-4-RR, Clone:240455D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83500-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83500-4-RR

    Affinity and Kinetic Characterization of 83500-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83500-4-RR against Human C6orf130 were performed. The affinity constant is below 1 pM.

C6orf130 Recombinant monoclonal antibody, PBS Only

C6orf130 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83500-4-PBS
Clone No.240455D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

OARD1, [Protein ADP-ribosylglutamate] hydrolase OARD1, 240455D7, ADP-ribose glycohydrolase OARD1, EC:3.2.2.-

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83500-4-PBS targets C6orf130 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19341

Product name: Recombinant human C6orf130 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-152 aa of BC011709

Sequence: MASSLNEDPEGSRITYVKGDLFACPKTDSLAHCISEDCRMGAGIAVLFKKKFGGVQELLNQQKKSGEVAVLKRDGRYIYYLITKKRASHKPTYENLQKSLEAMKSHCLKNGVTDLSMPRIGCGLDRLQWENVSAMIEEVFEATDIKITVYTL

相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 130
Calculated molecular weight 152 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC011709
Gene Symbol C6orf130
Gene ID (NCBI) 221443
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y530
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

OARD1 (O-acetyl-ADP-ribose deacetylase 1), also known as TARG1 (Terminal ADP-ribose protein glycohydrolase 1) and C6orf130, is a ADP-ribose glycohydrolase that hydrolyzes ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on glutamate and O-acetyl-ADP-D-ribose (PMID: 21849506; 23474714; 23481255). The MW of this protein is 17 kDa, and this antibody specially recognises the 17 kDa protein.

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