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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C6orf130 Recombinant antibody (83500-4-RR)

    WB analysis using 83500-4-RR (same clone as 83500-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83500-4-RR (C6orf130 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83500-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using C6orf130 Recombinant antibody (83500-4-RR)

    FC experiment of A431 using 83500-4-RR (same clone as 83500-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug C6orf130 Recombinant antibody (83500-4-RR, Clone:240455D7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83500-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using C6orf130 Recombinant antibody (83500-4-RR)

    FC experiment of PC-3 using 83500-4-RR (same clone as 83500-4-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug C6orf130 Recombinant antibody (83500-4-RR, Clone:240455D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83500-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83500-4-RR

    Affinity and Kinetic Characterization of 83500-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83500-4-RR against Human C6orf130 were performed. The affinity constant is below 1 pM.

C6orf130 Recombinant monoclonal antibody, PBS Only

C6orf130 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83500-4-PBS
Clone No.240455D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

OARD1, [Protein ADP-ribosylglutamate] hydrolase OARD1, 240455D7, ADP-ribose glycohydrolase OARD1, EC:3.2.2.-

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83500-4-PBS targets C6orf130 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19341

Product name: Recombinant human C6orf130 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-152 aa of BC011709

Sequence: MASSLNEDPEGSRITYVKGDLFACPKTDSLAHCISEDCRMGAGIAVLFKKKFGGVQELLNQQKKSGEVAVLKRDGRYIYYLITKKRASHKPTYENLQKSLEAMKSHCLKNGVTDLSMPRIGCGLDRLQWENVSAMIEEVFEATDIKITVYTL

相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 130
Calculated molecular weight 152 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC011709
Gene Symbol C6orf130
Gene ID (NCBI) 221443
RRIDAB_3671126
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y530
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

OARD1 (O-acetyl-ADP-ribose deacetylase 1), also known as TARG1 (Terminal ADP-ribose protein glycohydrolase 1) and C6orf130, is a ADP-ribose glycohydrolase that hydrolyzes ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on glutamate and O-acetyl-ADP-D-ribose (PMID: 21849506; 23474714; 23481255). The MW of this protein is 17 kDa, and this antibody specially recognises the 17 kDa protein.

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