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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C6orf108 Monoclonal antibody (68310-1-Ig)

    WB analysis using 68310-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68310-1-Ig (C6orf108 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C6orf108 Monoclonal antibody (68310-1-Ig)

    IF Staining of HEK-293 using 68310-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C6orf108 antibody (68310-1-Ig, Clone: 2C4E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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C6orf108 Monoclonal antibody

C6orf108 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68310-1-Ig
Clone No.2C4E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

DNPH1, EC:3.2.2.-, c-Myc-responsive protein RCL, c Myc responsive protein Rcl, 5-hydroxymethyl-dUMP N-hydrolase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68310-1-Ig targets C6orf108 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen C6orf108 fusion protein Ag22382 相同性解析による交差性が予測される生物種
Full Name chromosome 6 open reading frame 108
Calculated molecular weight 174 aa, 19 kDa
Observed molecular weight23 kDa
GenBank accession numberBC011683
Gene Symbol C6orf108
Gene ID (NCBI) 10591
RRIDAB_2935389
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43598
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C6orf108 also named as DNPH1, catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases (PMID: 24260472). Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers (PMID: 18726892).

Protocols

Product Specific Protocols
WB protocol for C6orf108 antibody 68310-1-IgDownload protocol
IF protocol for C6orf108 antibody 68310-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols