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Validation Data Gallery

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  • Western Blot (WB) analysis of human blood using Complement C6 Polyclonal antibody (17239-1-AP)

    WB analysis of human blood using 17239-1-AP (same clone as 17239-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 17239-1-AP (C6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using Complement C6 Polyclonal antibody (17239-1-AP)

    IP experiment of human plasma using 17239-1-AP (same clone as 17239-1-PBS)

    IP result of anti-Complement C6 (IP:17239-1-AP, 4ug; Detection:17239-1-AP 1:3000) with human plasma lysate 3200 ug. This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Complement C6 Polyclonal antibody (17239-1-AP)

    IHC staining of mouse liver using 17239-1-AP (same clone as 17239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Complement C6 Polyclonal antibody (17239-1-AP)

    IHC staining of mouse liver using 17239-1-AP (same clone as 17239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Complement C6 Polyclonal antibody (17239-1-AP)

    IHC staining of human liver cancer using 17239-1-AP (same clone as 17239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17239-1-AP (C6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Complement C6 Polyclonal antibody (17239-1-AP)

    IHC staining of human liver cancer using 17239-1-AP (same clone as 17239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17239-1-AP (C6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

Complement C6 Polyclonal antibody, PBS Only

Complement C6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17239-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

C6, complement component 6, Complement component C6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17239-1-PBS targets Complement C6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11083

Product name: Recombinant human C6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 586-934 aa of BC035723

Sequence: TRECNNPAPQRGGKRCEGEKRQEEDCTFSIMENNGQPCINDDEEMKEVDLPEIEADSGCPQPVPPENGFIRNEKQLYLVGEDVEISCLTGFETVGYQYFRCLPDGTWRQGDVECQRTECIKPVVQEVLTITPFQRLYRIGESIELTCPKGFVVAGPSRYTCQGNSWTPPISNSLTCEKDTLTKLKGHCQLGQKQSGSECICMSPEEDCSHHSEDLCVFDTDSNDYFTSPACKFLAEKCLNNQQLHFLHIGSCQDGRQLEWGLERTRLSSNSTKKESCGYDTCYDWEKCSASTSKCVCLLPPQCFKGGNQLYCVKMGSSTSEKTLNICEVGTIRCANRKMEILHPGKCLA

相同性解析による交差性が予測される生物種
Full Name complement component 6
Calculated molecular weight 934 aa, 105 kDa
Observed molecular weight105-120 kDa
GenBank accession numberBC035723
Gene Symbol C6
Gene ID (NCBI) 729
RRIDAB_2878366
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP13671
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The final consequence of complement activation is formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. C6 is 1 of 5 late-acting proteins of complement that participate in assembly and function of the MAC. The deduced C6 protein contains 934 amino acids, including a 21-amino acid N-terminal signal sequence and 2 N-glycosylation sites. Mutations in the C6 gene are associated with complement component-6 deficiency.

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