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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C5orf51 Polyclonal antibody

C5orf51 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30107-1-AP

Synonyms

C5orf51, UPF0600 protein C5orf51

Validation Data Gallery

Applications:
  • WB analysis using 30107-1-AP

    WB analysis using 30107-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30107-1-AP (C5orf51 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 30107-1-AP

    WB analysis using 30107-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30107-1-AP (C5orf51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 30107-1-AP

    IHC staining of mouse liver using 30107-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30107-1-AP (C5orf51 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 30107-1-AP

    IHC staining of mouse liver using 30107-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30107-1-AP (C5orf51 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 30107-1-AP

    IF Staining of HeLa using 30107-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C5orf51 antibody (30107-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 30107-1-AP

    IF Staining of HeLa using 30107-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C5orf51 antibody (30107-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, HepG2 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

30107-1-AP targets C5orf51 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5orf51 fusion protein Ag32440 相同性解析による交差性が予測される生物種
Full Name chromosome 5 open reading frame 51
Calculated molecular weight34kd
Observed molecular weight34-40 kDa
GenBank accession numberXM_011514032
Gene symbol C5orf51
Gene ID (NCBI) 285636
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C5orf51 antibody 30107-1-APDownload protocol
IHC protocol for C5orf51 antibody 30107-1-APDownload protocol
IF protocol for C5orf51 antibody 30107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols