• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C5orf33 Polyclonal antibody

C5orf33 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26352-1-AP

Synonyms

C5orf33, NAD kinase 2, NADK2, UPF0465 protein C5orf33

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 26352-1-AP

    WB analysis of mouse liver using 26352-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26352-1-AP (C5orf33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 26352-1-AP

    WB analysis of HL-60 using 26352-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26352-1-AP (C5orf33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 26352-1-AP

    IHC staining of human liver using 26352-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26352-1-AP (C5orf33 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 26352-1-AP

    IHC staining of human liver using 26352-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26352-1-AP (C5orf33 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 26352-1-AP

    IF Staining of HeLa using 26352-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26352-1-AP (C5orf33 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue, HL-60 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26352-1-AP is C5orf33 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C5orf33 fusion protein Ag23706 相同性解析による交差性が予測される生物種
Full Name chromosome 5 open reading frame 33
Observed molecular weight 40-50 kDa
GenBank accession numberBC062567
Gene symbol C5orf33
Gene ID (NCBI) 133686
RRIDAB_2880487
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C5orf33 antibody 26352-1-APDownload protocol
IHC protocol for C5orf33 antibody 26352-1-APDownload protocol
IF protocol for C5orf33 antibody 26352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FASEB J

G-protein-coupled estrogen receptor 1 promotes peritoneal metastasis of gastric cancer through nicotinamide adenine dinucleotide kinase 1-mediated redox modulation

Authors - Teng Wu
View Article