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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C5a Recombinant monoclonal antibody (86463-4-RR)

    WB analysis of human plasma using 86463-4-RR

    human plasma was subjected to SDS PAGE followed by western blot with 86463-4-RR (Complement C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity of 86463-4-RR

    Affinity of 86463-4-RR

    Biolayer interferometry (BLI) kinetic assays of 86463-4-RR against Human C5a were performed. The affinity constant is 0.251 nM.

C5a Recombinant monoclonal antibody

C5a Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86463-4-RR
Clone No.251190F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, complement component 5, CPAMD4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86463-4-RR targets C5a in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg4205

Product name: Recombinant Human Complement C5 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 678-751 aa of NM_001735.3

Sequence: TLQKKIEEIAAKYKHSVVKKCCYDGACVNNDETCEQRAARISLGPRCIKAFTECCVVASQLRANISHKDMQLGR

相同性解析による交差性が予測される生物種
Full Name complement component 5
Calculated molecular weight188 kDa
Observed molecular weight120 kDa
GenBank accession numberNM_001735.3
Gene Symbol C5
Gene ID (NCBI) 727
RRIDAB_3744903
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. This antibody specifically recognizes the C5 alpha chain.(PMID: 20010915; PMID: 8011297; PMID: 6554279)

Protocols

Product Specific Protocols
WB protocol for C5a antibody 86463-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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