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Validation Data Gallery

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  • Western Blot (WB) analysis of A2780 cells using C4orf49 Polyclonal antibody (22569-1-AP)

    WB analysis of A2780 using 22569-1-AP (same clone as 22569-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22569-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SKOV-3 cells using C4orf49 Polyclonal antibody (22569-1-AP)

    WB analysis of SKOV-3 using 22569-1-AP (same clone as 22569-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22569-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using C4orf49 Polyclonal antibody (22569-1-AP)

    WB analysis of A549 using 22569-1-AP (same clone as 22569-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 22569-1-AP (C4orf49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using C4orf49 Polyclonal antibody (22569-1-AP)

    IHC staining of human testis using 22569-1-AP (same clone as 22569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using C4orf49 Polyclonal antibody (22569-1-AP)

    IHC staining of human ovary using 22569-1-AP (same clone as 22569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using C4orf49 Polyclonal antibody (22569-1-AP)

    IHC staining of human testis using 22569-1-AP (same clone as 22569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22569-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using C4orf49 Polyclonal antibody (22569-1-AP)

    IHC staining of human ovary using 22569-1-AP (same clone as 22569-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary slide using 22569-1-AP (C4orf49 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22569-1-PBS in a different storage buffer formulation.

C4orf49 Polyclonal antibody, PBS Only

C4orf49 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22569-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22569-1-PBS targets C4orf49 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16552

Product name: Recombinant human C4orf49 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-240 aa of BC104174

Sequence: MYLRRAVSKTLALPLRAPPNPAPLGKDASLRRMSSNRFPGSSGSNMIYYLVVGVTVSAGGYYAYKTVTSDQAKHTEHKTNLKEKTKAEIHPFQGEKENVAETEKASSEAPEELIVEAEVVDAEESPSATVVVIKEASACPGHVEAAPETTAVSAETGPEVTDAAARETTEVNPETTPEVTNAALDEAVTIDNDKDTTKNETSDEYAELEEENSPAESESSAGDDLQEEASVGSEAASAQG

相同性解析による交差性が予測される生物種
Full Name chromosome 4 open reading frame 49
Calculated molecular weight 240 aa, 25 kDa
Observed molecular weight 33-36 kDa
GenBank accession numberBC104174
Gene Symbol C4orf49
Gene ID (NCBI) 84709
RRIDAB_2879124
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDB4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The protein MGARP, also named as C4ORF49, CESP1, HUMMR, OSAP and GS3582, plays a role in the trafficking of mitochondria along microtubules. It regulates the kinesin-mediated axonal transport of mitochondria to nerve terminals along microtubules during hypoxia. MGARP is localized to the mitochondria in cultured human CE, and its N-terminal seems to be processed after mitochondrial import. The MW of MGARP is about 33-36 kDa(PMID: 16565373).

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