• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HAUS3 Polyclonal antibody

HAUS3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18991-1-AP

Synonyms

C4orf15, HAUS3, IT1

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 18991-1-AP

    WB analysis of HepG2 using 18991-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18991-1-AP (HAUS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18991-1-AP

    WB analysis of HeLa using 18991-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18991-1-AP (HAUS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 18991-1-AP

    IHC staining of human liver cancer using 18991-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18991-1-AP (HAUS3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver cancer using 18991-1-AP

    IHC staining of human liver cancer using 18991-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18991-1-AP (HAUS3 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 18991-1-AP

    IF Staining of HepG2 using 18991-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18991-1-AP (HAUS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

18991-1-AP targets HAUS3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name chromosome 4 open reading frame 15
Calculated molecular weight 70 kDa
Observed molecular weight 62-65 kDa
GenBank accession numberNM_024511
Gene symbol C4orf15
Gene ID (NCBI) 79441
RRIDAB_2301967
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HAUS3, also named as C4orf15, belongs to the HAUS3 family. It is Required for mitotic centrosome and spindle integrity. HAUS3 is a component of the HAUS augmin-like complex which regulates mitotic spindle assembly and centrosome integrity and is required for completion of cytokinesis. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly.

Protocols

Product Specific Protocols
WB protocol for HAUS3 antibody 18991-1-APDownload protocol
IHC protocol for HAUS3 antibody 18991-1-APDownload protocol
IF protocol for HAUS3 antibody 18991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article