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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using HAUS3 Polyclonal antibody (18991-1-AP)

    WB analysis of HepG2 using 18991-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18991-1-AP (HAUS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HAUS3 Polyclonal antibody (18991-1-AP)

    WB analysis of HeLa using 18991-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18991-1-AP (HAUS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HAUS3 Polyclonal antibody (18991-1-AP)

    IHC staining of human liver cancer using 18991-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18991-1-AP (HAUS3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using HAUS3 Polyclonal antibody (18991-1-AP)

    IHC staining of human liver cancer using 18991-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18991-1-AP (HAUS3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using HAUS3 Polyclonal antibody (18991-1-AP)

    IHC staining of human urothelial carcinoma using 18991-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 18991-1-AP (HAUS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HAUS3 Polyclonal antibody (18991-1-AP)

    IF Staining of HepG2 using 18991-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HAUS3 antibody (18991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HAUS3 Polyclonal antibody (18991-1-AP)

    IF Staining of HeLa using 18991-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HAUS3 antibody (18991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

HAUS3 Polyclonal antibody

HAUS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18991-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C4orf15, HAUS augmin-like complex subunit 3, IT1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman urothelial carcinoma tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18991-1-AP targets HAUS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name chromosome 4 open reading frame 15
Calculated molecular weight 70 kDa
Observed molecular weight 62-65 kDa
GenBank accession numberNM_024511
Gene Symbol HAUS3
Gene ID (NCBI) 79441
RRIDAB_2301967
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ68CZ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HAUS3, also named as C4orf15, belongs to the HAUS3 family. It is Required for mitotic centrosome and spindle integrity. HAUS3 is a component of the HAUS augmin-like complex which regulates mitotic spindle assembly and centrosome integrity and is required for completion of cytokinesis. The complex interacts with the gamma-tubulin ring complex and this interaction is required for spindle assembly.

Protocols

Product Specific Protocols
IF protocol for HAUS3 antibody 18991-1-APDownload protocol
IHC protocol for HAUS3 antibody 18991-1-APDownload protocol
WB protocol for HAUS3 antibody 18991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article
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