Validation Data Gallery
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Tested Applications
| Positive WB detected in | human blood |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85782-2-RR targets C4b Alpha Chain/C4b-B/C4d-B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | complement component 4B (Chido blood group) |
| Calculated molecular weight | 193 kDa |
| Observed molecular weight | 95 kDa |
| GenBank accession number | NM_000592 |
| Gene Symbol | C4B |
| Gene ID (NCBI) | 721 |
| RRID | AB_3744369 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P0C0L5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single-chain precursor, which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1116-1128aa of human C4b protein can recognize C4b precursor, C4b alpha chain, C4b-B, and C4d-B.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for C4b Alpha Chain/C4b-B/C4d-B antibody 85782-2-RR | Download protocol |
| WB protocol for C4b Alpha Chain/C4b-B/C4d-B antibody 85782-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |