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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C4 Gamma Chain Polyclonal antibody (17160-1-AP)

    WB analysis of human plasma using 17160-1-AP (same clone as 17160-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17160-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using C4 Gamma Chain Polyclonal antibody (17160-1-AP)

    WB analysis of HepG2 using 17160-1-AP (same clone as 17160-1-PBS)

    WB result of C4 Gamma Chain antibody (17160-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C4 Gamma Chain transfected HepG2 cells. This data was developed using the same antibody clone with 17160-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C4 Gamma Chain Polyclonal antibody (17160-1-AP)

    IHC staining of human liver cancer using 17160-1-AP (same clone as 17160-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17160-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C4 Gamma Chain Polyclonal antibody (17160-1-AP)

    IHC staining of human liver cancer using 17160-1-AP (same clone as 17160-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17160-1-AP (C4 Gamma Chain antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17160-1-PBS in a different storage buffer formulation.

C4 Gamma Chain Polyclonal antibody, PBS Only

C4 Gamma Chain Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17160-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

C4 (gamma chain), C4A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17160-1-PBS targets C4 Gamma Chain in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10449

Product name: Recombinant human C4A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1543-1744 aa of NM_001252204

Sequence: EVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEVCQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREGSRAAFRLFETKITQVLHFTKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLESNSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV

相同性解析による交差性が予測される生物種
Full Name complement component 4A (Rodgers blood group)
Calculated molecular weight 1744 aa, 193 kDa
Observed molecular weight~190 kDa, 30-35 kDa
GenBank accession numberNM_001252204
Gene Symbol C4 Gamma Chain
Gene ID (NCBI) 720
RRIDAB_2923565
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP0C0L4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single-chain precursor that is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular weights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody, raised against 1543-1744aa of human C4A protein, recognizes the C4 gamma chain.

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