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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C3orf62 Polyclonal antibody (32718-1-AP)

    WB analysis using 32718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32718-1-AP (C3orf62 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C3orf62 Polyclonal antibody (32718-1-AP)

    IHC staining of mouse testis using 32718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32718-1-AP (C3orf62 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using C3orf62 Polyclonal antibody (32718-1-AP)

    IHC staining of mouse testis using 32718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32718-1-AP (C3orf62 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using C3orf62 Polyclonal antibody (32718-1-AP)

    IHC staining of rat testis using 32718-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 32718-1-AP (C3orf62 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using C3orf62 Polyclonal antibody (32718-1-AP)

    IHC staining of rat testis using 32718-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 32718-1-AP (C3orf62 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C3orf62 Polyclonal antibody

C3orf62 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32718-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Uncharacterized protein C3orf62

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32718-1-AP targets C3orf62 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag37728

Product name: Recombinant human C3orf62 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-267 aa of BC023586

Sequence: MHYIKTWSLLGEMSEKLRRCRKELTAAIDRAFEGVSYSQECTGQQRLELSAAPLSFSLPVHRLLCRRHPLAACSSAAPFAAVPCAPENENPAFATNHAPVNAKPHALCPERKPLTSKENVLMHSSILAPERESWRTAGEGENWRKENLRKDMERDLKADSNMPLNNSSQEVTKDLLDMIDHTSIRTIEELAGKIEFENELNHMCGHCQDSPFKEEAWALLMDKSPQKATDADPGSLKQAFDDHNIVETVLDLEEDYNVMTSFKYQIE

相同性解析による交差性が予測される生物種
Full Name chromosome 3 open reading frame 62
Calculated molecular weight30 kDa
Observed molecular weight38 kDa
GenBank accession numberBC023586
Gene Symbol C3orf62
Gene ID (NCBI) 375341
RRIDAB_3742721
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ6ZUJ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Arrest-type spermatogenic failure refers to the arrest of spermatogenesis at a specific stage, such as spermatogonia, spermatocytes, or spermatids. It is mainly caused by genetic factors, particularly variants in key spermatogenic genes (e.g., TEX11, MSH5, C3ORF62, MEI1, etc.), and represents one of the most distinctive forms of male infertility (PMID: 41669890). The C3orf62 is essential for normal spermatogenesis and male fertility (PMID: 33064489; 38175751).

Protocols

Product Specific Protocols
IHC protocol for C3orf62 antibody 32718-1-APDownload protocol
WB protocol for C3orf62 antibody 32718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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