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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using C3orf43 Polyclonal antibody (25362-1-AP)

    WB analysis of mouse heart using 25362-1-AP (same clone as 25362-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25362-1-AP (C3orf43 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25362-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using C3orf43 Polyclonal antibody (25362-1-AP)

    WB analysis of human plasma using 25362-1-AP (same clone as 25362-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 25362-1-AP (C3orf43 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25362-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C3orf43 Polyclonal antibody (25362-1-AP)

    IF Staining of A431 using 25362-1-AP (same clone as 25362-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C3orf43 antibody (25362-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25362-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C3orf43 Polyclonal antibody (25362-1-AP)

    IF Staining of A431 using 25362-1-AP (same clone as 25362-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using C3orf43 antibody (25362-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25362-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C3orf43 Polyclonal antibody (25362-1-AP)

    IF Staining of HeLa using 25362-1-AP (same clone as 25362-1-PBS)

    Immunofluorescent analysis of HeLa cells using 25362-1-AP (C3orf43 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 25362-1-PBS in a different storage buffer formulation.

C3orf43 Polyclonal antibody, PBS Only

C3orf43 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 25362-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Single-pass membrane and coiled-coil domain-containing protein 1, Single-pass membrane protein with coiled-coil domains 1, SMCO1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25362-1-PBS targets C3orf43 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21670

Product name: Recombinant human C3orf43 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-214 aa of BC118636

Sequence: MNNETTTLISLKEAMKRVDHKLQALETQFKELDFTKDNLMQKFEHHSKALASQAAQDEMWTAVRALQLTSMELNILYSYVIEVLICLHTRVLEKLPDLVRGLPTLASVLRRKVKNKRVRVVWESILEECGLQEGDITALCTFFIARGNKAEHYTAKVRQMYIRDVTFLITNMVKNQALQDSLLRAVQVIEKGKAVRTPEKQKSSLEELIPSVKN

相同性解析による交差性が予測される生物種
Full Name chromosome 3 open reading frame 43
Calculated molecular weight 214 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC118636
Gene Symbol C3orf43
Gene ID (NCBI) 255798
RRIDAB_2880044
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ147U7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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