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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C2orf69 Polyclonal antibody (32100-1-AP)

    WB analysis using 32100-1-AP (same clone as 32100-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32100-1-AP (C2orf69 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32100-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using C2orf69 Polyclonal antibody (32100-1-AP)

    IHC staining of mouse kidney using 32100-1-AP (same clone as 32100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32100-1-AP (C2orf69 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32100-1-PBS in a different storage buffer formulation.

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C2orf69 Polyclonal antibody, PBS Only

C2orf69 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 32100-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Chromosome 2 Open Reading Frame 69, COXPD53, FLJ38973, Hypothetical Protein FLJ38973, Mitochondrial protein C2orf69

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

32100-1-PBS targets C2orf69 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C2orf69 fusion protein Ag36779 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 69
Calculated molecular weight43 kDa
Observed molecular weight50 kDa
GenBank accession numberBC036456
Gene Symbol C2orf69
Gene ID (NCBI) 205327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8N8R5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Chromosome 2 open reading frame 69 (C2orf69, also known as COXPD53) is located in the mitochondrion. It can affect mitochondrial membrane potential and oxidative respiration in cultured neurons and controls the levels of the glycogen branching enzyme 1 (GBE1) (PMID: 34038740; 33945503). The variant in C2orf69 probably causes developmental regression, seizures, microcephaly, autistic features, and hypertonia (PMID: 37337918). Its deficiency can disrupt the development and homeostasis of the immune and central nervous systems (PMID: 34038740).