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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C2orf49 Polyclonal antibody

C2orf49 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 18272-1-AP

Synonyms

Ashwin, ASW, C2orf49

Validation Data Gallery

Applications:
  • WB analysis using 18272-1-AP

    WB analysis using 18272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18272-1-AP (C2orf49 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 18272-1-AP

    WB analysis of mouse liver using 18272-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18272-1-AP (C2orf49 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 18272-1-AP

    IF Staining of A431 using 18272-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 18272-1-AP (C2orf49 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, Jurkat cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18272-1-AP targets C2orf49 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C2orf49 fusion protein Ag12948 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 49
Calculated molecular weight 26 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC001310
Gene symbol C2orf49
Gene ID (NCBI) 79074
RRIDAB_2066525
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C2orf49 antibody 18272-1-APDownload protocol
IF protocol for C2orf49 antibody 18272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols