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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using C2orf47 Polyclonal antibody (24930-1-AP)

    WB analysis of mouse kidney using 24930-1-AP (same clone as 24930-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24930-1-AP (C2orf47 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24930-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using C2orf47 Polyclonal antibody (24930-1-AP)

    WB analysis of PC-3 using 24930-1-AP (same clone as 24930-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24930-1-AP (C2orf47 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24930-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using C2orf47 Polyclonal antibody (24930-1-AP)

    WB analysis of HeLa using 24930-1-AP (same clone as 24930-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24930-1-AP (C2orf47 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24930-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using C2orf47 Polyclonal antibody (24930-1-AP)

    IHC staining of human kidney using 24930-1-AP (same clone as 24930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24930-1-AP (C2orf47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24930-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using C2orf47 Polyclonal antibody (24930-1-AP)

    IHC staining of human kidney using 24930-1-AP (same clone as 24930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24930-1-AP (C2orf47 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24930-1-PBS in a different storage buffer formulation.

C2orf47 Polyclonal antibody, PBS Only

C2orf47 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24930-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

m-AAA protease-interacting protein 1, mitochondrial, MAIP1, Matrix AAA peptidase-interacting protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24930-1-PBS targets C2orf47 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21518

Product name: Recombinant human C2orf47 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-291 aa of BC017959

Sequence: MALAARLLPQFLHSRSLPCGAVRLRTPAVAEVRLPSATLCYFCRCRLGLGAALFPRSARALAASALPAQGSRWPVLSSPGLPAAFASFPACPQRSYSTEEKPQQHQKTKMIVLGFSNPINWVRTRIKAFLIWAYFDKEFSITEFSEGAKQAFAHVSKLLSQCKFDLLEELVAKEVLHALKEKVTSLPDNHKNALAANIDEIVFTSTGDISIYYDEKGRKFVNILMCFWYLTSANIPSETLRGASVFQVKLGNQNVETKQLLSASYEFQREFTQGVKPDWTIARIEHSKLLE

相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 47
Calculated molecular weight 291 aa, 33 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC017959
Gene Symbol C2orf47
Gene ID (NCBI) 79568
RRIDAB_2879804
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWC4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C2orf47, also named matrix AAA peptidase interacting protein 1 (MAIP1), is a 291 amino-acid protein that localizes in the Mitochondrion. C2orf47 is involved in protecting EMRE, a component of the single calcium complex (MCU), from proteolysis by assisting membrane insertion.

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