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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C2orf37 Polyclonal antibody

C2orf37 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26033-1-AP

Synonyms

C2orf37, DCAF17

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 26033-1-AP

    WB analysis of HEK-293 using 26033-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26033-1-AP (C2orf37 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 26033-1-AP

    WB analysis of HL-60 using 26033-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26033-1-AP (C2orf37 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 26033-1-AP

    IHC staining of mouse liver using 26033-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26033-1-AP (C2orf37 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 26033-1-AP

    IHC staining of mouse liver using 26033-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26033-1-AP (C2orf37 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HL-60 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26033-1-AP is C2orf37 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C2orf37 fusion protein Ag21995 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 37
Calculated molecular weight 520 aa, 59 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC140843
Gene symbol C2orf37
Gene ID (NCBI) 80067
RRIDAB_2880344
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C2orf37 antibody 26033-1-APDownload protocol
IHC protocol for C2orf37 antibody 26033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols