Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 19809-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 19809-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 19809-1-AP
IP result of anti-RtcB-Specific (IP:19809-1-AP, 3ug; Detection:19809-1-AP 1:500) with HeLa cells lysate 3000ug.
IP experiment of HeLa using 19809-1-AP
IP result of anti-RtcB-Specific (IP:19809-1-AP, 4ug; Detection:19809-1-AP 1:4000) with HeLa cells lysate 1800 ug.
IHC staining of human lung cancer using 19809-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 19809-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 19809-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 19809-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RTCB-Specific antibody (19809-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of U2OS using 19809-1-AP
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RTCB-Specific antibody (19809-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HeLa using 19809-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.25 ug RtcB-Specific Polyclonal antibody (19809-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of A431 using 19809-1-AP
1x10^6 A431 cells were intracellularly stained with 0.25 ug RtcB-Specific Polyclonal antibody (19809-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

RTCB-Specific Polyclonal antibody

RTCB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19809-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HSPC117, RTCB, 3'-phosphate/5'-hydroxy nucleic acid ligase, C22orf28, EC:6.5.1.8

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human lung cancer tissue, human liver cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	A431 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IP	See 2 publications below

Product Information

19809-1-AP targets RTCB-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13836 Product name: Recombinant human C22orf28 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 159-505 aa of BC000151 Sequence: GVIPMNAKDLEEALEMGVDWSLREGYAWAEDKEHCEEYGRMLQADPNKVSARAKKRGLPQLGTLGAGNHYAEIQVVDEIFNEYAAKKMGIDHKGQVCVMIHSGSRGLGHQVATDALVAMEKAMKRDKIIVNDRQLACARIASPEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFAKVFNTTPDDFDLHVIYDVSHNIAKVEQHVVDGKERTLLVHRKGSTRAFPPHHPLIAVDYQLTGQPVLIGGTMGTCSYVLTGTEQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEAPESYKNVTDVVNTCHDAGISKKAIKLRPIAVIKG 相同性解析による交差性が予測される生物種
Full Name	chromosome 22 open reading frame 28
Calculated molecular weight	55 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC000151
Gene Symbol	RTCB
Gene ID (NCBI)	51493
RRID	AB_10695047
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y3I0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RTCB (also known as HSPC117, C22orf28, FAAP, and D10Wsu52e) is a essential subunit of the tRNA ligase complex, which is localized in both the cytoplasm and nucleus, and is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB also serves as a cell adhesion protein and plays an important role in embryo and placenta development. Recently, RTCB has been identified as an UPR RNA ligase that catalyzes unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)

Protocols

Product Specific Protocols
WB protocol for RTCB-Specific antibody 19809-1-AP	Download protocol
IHC protocol for RTCB-Specific antibody 19809-1-AP	Download protocol
IF protocol for RTCB-Specific antibody 19809-1-AP	Download protocol
IP protocol for RTCB-Specific antibody 19809-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance. Authors - Yichen Xu View Article
human	WB,IP	Mol Cell A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB. Authors - Yanyan Lu View Article
pig	WB	Nat Commun Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells. Authors - Carmen Aguilar View Article
human	WB	J Biol Chem The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress. Authors - Irem Unlu KO Validated View Article
human	IP	Life Sci Alliance Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs. Authors - Alexandra Papaioannou View Article
human	WB	J Immunol The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1 Authors - Lei Cao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 19809-1-AP

WB analysis of mouse brain using 19809-1-AP

IP experiment of HeLa using 19809-1-AP

IP experiment of HeLa using 19809-1-AP

IHC staining of human lung cancer using 19809-1-AP

IHC staining of human stomach cancer using 19809-1-AP

IHC staining of human liver cancer using 19809-1-AP

IF Staining of HeLa using 19809-1-AP

IF Staining of U2OS using 19809-1-AP

FC experiment of HeLa using 19809-1-AP

FC experiment of A431 using 19809-1-AP

RTCB-Specific Polyclonal antibody

RTCB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.

A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB.

Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells.

The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.

Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs.

The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1