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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using C21orf66 Polyclonal antibody (21357-1-AP)

    WB analysis of human brain using 21357-1-AP (same clone as 21357-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 21357-1-AP (C21orf66 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using C21orf66 Polyclonal antibody (21357-1-AP)

    IHC staining of human pancreas cancer using 21357-1-AP (same clone as 21357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using C21orf66 Polyclonal antibody (21357-1-AP)

    IHC staining of human renal cell carcinoma using 21357-1-AP (same clone as 21357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using C21orf66 Polyclonal antibody (21357-1-AP)

    IHC staining of human renal cell carcinoma using 21357-1-AP (same clone as 21357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C21orf66 Polyclonal antibody (21357-1-AP)

    IHC staining of mouse liver using 21357-1-AP (same clone as 21357-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C21orf66 Polyclonal antibody (21357-1-AP)

    IF Staining of HeLa using 21357-1-AP (same clone as 21357-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C21orf66 antibody (21357-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21357-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21357-1-PBS targets C21orf66 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C21orf66 fusion protein Ag15789 相同性解析による交差性が予測される生物種
Full Name chromosome 21 open reading frame 66
Calculated molecular weight 917 aa, 105 kDa
Observed molecular weight 93 kDa
GenBank accession numberBC030539
Gene Symbol C21orf66
Gene ID (NCBI) 94104
RRIDAB_10733239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5B6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.