Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Hela cells, HepG2 cells, K-562 cells, THP-1 cells, mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32198-1-AP targets C21orf33 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37527 Product name: Recombinant human C21orf33 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 42-268 aa of BC002370 Sequence: AARVALVLSGCGVYDGTEIHEASAILVHLSRGGAEVQIFAPDVPQMHVIDHTKGQPSEGESRNVLTESARIARGKITDLANLSAANHDAAIFPGGFGAAKNLSTFAVDGKDCKVNKEVERVLKEFHQAGKPIGLCCIAPVLAAKVLRGVEVTVGHEQEEGGKWPYAGTAEAIKALGAKHCVKEVVEAHVDQKNKVVTTPAFMCETALHYIHDGIGAMVRKVLELTGK 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 21 open reading frame 33 |
| Calculated molecular weight | 268 aa, 28 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC002370 |
| Gene Symbol | C21orf33 |
| Gene ID (NCBI) | 8209 |
| RRID | AB_3670221 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P0DPI2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GATD3A is a member of glutamine amidotransferase-like class 1 domain-containing (GATD) family. GATD proteins classically generate ammonia by amidolysis from the glutamine amide nitrogen, transferring it to an acceptor substrate in a variety of anabolic reactions involving purine and pyrimidine synthesis (PMID: 9575335). GATD3A restricts the formation of AGEs in mitochondria and is a relevant target for diseases where AGE deposition is a pathological hallmark (PMID: 35307029).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C21orf33 antibody 32198-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |