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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C21orf2 Polyclonal antibody (27609-1-AP)

    WB analysis using 27609-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using C21orf2 Polyclonal antibody (27609-1-AP)

    WB analysis of HEK-293T using 27609-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using C21orf2 Polyclonal antibody (27609-1-AP)

    WB analysis of HEK-293T using 27609-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using human C21orf2 Polyclonal antibody (27609-1-AP)

    IP experiment of HEK-293T using 27609-1-AP

    IP result of anti-C21orf2 (IP:27609-1-AP, 4ug; Detection:27609-1-AP 1:3000) with HEK-293T cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using C21orf2 Polyclonal antibody (27609-1-AP)

    IHC staining of human lung cancer using 27609-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27609-1-AP (C21orf2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using C21orf2 Polyclonal antibody (27609-1-AP)

    IHC staining of human lung cancer using 27609-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27609-1-AP (C21orf2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C21orf2 Polyclonal antibody

C21orf2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 27609-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

A2, C21orf-HUMF09G8.5, CFAP410, Cilia- and flagella-associated protein 410, Leucine-rich repeat-containing protein 76

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27609-1-AP targets C21orf2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26470

Product name: Recombinant human C21orf2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-256 aa of BC072012

Sequence: MKLTRKMVLTRAKASELHSVRKLNCWGSRLTDISICQEMPSLEVITLSVNSISTLEPVSRCQRLSELYLRRNRIPSLAELFYLKGLPRLRVLWLAENPCCGTSPHRYRMTVLRTLPRLQKLDNQAVTEEELSRALSEGEEITAAPEREGTGHGGPKLCCTLSSLSSAAETGRDPLDSEEEATSGAQDERGLKPPSRGQFPSLSARDASSSHRGRNVLTAILLLLRELDAEGLEAVQQTVGSRLQALRGEEVQEHAE

相同性解析による交差性が予測される生物種
Full Name chromosome 21 open reading frame 2
Observed molecular weight 28-32 kDa
GenBank accession numberBC072012
Gene Symbol C21orf2
Gene ID (NCBI) 755
RRIDAB_2880924
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43822
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for C21orf2 antibody 27609-1-APDownload protocol
IP protocol for C21orf2 antibody 27609-1-APDownload protocol
WB protocol for C21orf2 antibody 27609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Neuropathol Commun

ALS-associated C21ORF2 variant disrupts DNA damage repair, mitochondrial metabolism, neuronal excitability and NEK1 levels in human motor neurons

Authors - Pavol Zelina
View Article
humanWB,IP

Life Sci Alliance

Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases

Authors - Mateusz Gregorczyk
View Article
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