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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RALGAPA2 Polyclonal antibody

RALGAPA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29843-1-AP

Synonyms

250 kDa substrate of Akt, AS250, bA287B20.1, C20orf74, dJ1049G11, dJ1049G11.4, FLJ12819, KIAA1272, p220, RALGAPA2

Validation Data Gallery

Applications:
  • WB analysis using 29843-1-AP

    WB analysis using 29843-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29843-1-AP (RALGAPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29843-1-AP

    WB analysis using 29843-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29843-1-AP (RALGAPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29843-1-AP

    IHC staining of mouse testis using 29843-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29843-1-AP (C20orf74 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 29843-1-AP

    IHC staining of mouse testis using 29843-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29843-1-AP (C20orf74 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HUVEC cells, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29843-1-AP targets RALGAPA2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RALGAPA2 fusion protein Ag31460 相同性解析による交差性が予測される生物種
Full Name chromosome 20 open reading frame 74
Calculated molecular weight211KD
Observed molecular weight230 kDa
GenBank accession numberNM_020343
Gene symbol C20orf74
Gene ID (NCBI) 57186
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RALGAPA2 antibody 29843-1-APDownload protocol
IHC protocol for RALGAPA2 antibody 29843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols