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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using C20orf20 Polyclonal antibody (26040-1-AP)

    WB analysis of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26040-1-AP (C20orf20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using C20orf20 Polyclonal antibody (26040-1-AP)

    WB analysis of mouse testis using 26040-1-AP (same clone as 26040-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26040-1-AP (C20orf20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using C20orf20 Polyclonal antibody (26040-1-AP)

    IP experiment of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

    IP result of anti-C20orf20 (IP:26040-1-AP, 4ug; Detection:26040-1-AP 1:300) with HepG2 cells lysate 2640ug. This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C20orf20 Polyclonal antibody (26040-1-AP)

    IHC staining of human colon cancer using 26040-1-AP (same clone as 26040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26040-1-AP (C20orf20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C20orf20 Polyclonal antibody (26040-1-AP)

    IHC staining of human colon cancer using 26040-1-AP (same clone as 26040-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26040-1-AP (C20orf20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C20orf20 Polyclonal antibody (26040-1-AP)

    IF Staining of HepG2 using 26040-1-AP (same clone as 26040-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C20orf20 antibody (26040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 26040-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

C20orf20 Polyclonal antibody, PBS Only

C20orf20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 26040-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Eaf7, MRG/MORF4L-binding protein, MRG15BP, MRG-binding protein, MRGBP

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26040-1-PBS targets C20orf20 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22840

Product name: Recombinant human C20orf20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-204 aa of BC014235

Sequence: MGEAEVGGGGAAGDKGPGEAATSPAEETVVWSPEVEVCLFHAMLGHKPVGVNRHFHMICIRDKFSQNIGRQVPSKVIWDHLSTMYDMQALHESEILPFPNPERNFVLPEEIIQEVREGKVMIEEEMKEEMKEDVDPHNGADDVFSSSGSLGKASEKSSKDKEKNSSDLGCKEGADKRKRSRVTDKVLTANSNPSSPSAAKRRRT

相同性解析による交差性が予測される生物種
Full Name chromosome 20 open reading frame 20
Calculated molecular weight 204 aa, 22 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC014235
Gene Symbol C20orf20
Gene ID (NCBI) 55257
RRIDAB_2880347
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NV56
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C20orf20, also named as MRG/MORF4L-binding protein or Up-regulated in colon cancer 4 (Urcc4), is a 204 amino acid protein, which belongs to the EAF7 family. C20orf20 localizes in the nucleus and is a Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. C20orf20 is modified through phosphorylation and acetylation, so the molecular weight of C20orf20 might be 25-30 kDa.

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