Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells, Raji cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
27175-1-AP targets Complement C2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Complement C2 fusion protein Ag25690 相同性解析による交差性が予測される生物種 |
| Full Name | complement component 2 |
| Observed molecular weight | 70 kDa, 80 kDa |
| GenBank accession number | BC043484 |
| Gene Symbol | C2 |
| Gene ID (NCBI) | 717 |
| RRID | AB_2880787 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06681 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Complement C2 (C2) is a key component of the classical and lectin pathways of the complement system. Complement C2 deficiency is associated with an increased susceptibility to infections, particularly those caused by encapsulated bacteria.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Complement C2 antibody 27175-1-AP | Download protocol |
| IHC protocol for Complement C2 antibody 27175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Gastroenterol Hepatol A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function. | ||
Insect Biochem Mol Biol Sequence analysis and function of mosquito aeCCC2 and Drosophila Ncc83 orthologs. |