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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1orf83 Recombinant antibody (83626-5-RR)

    WB analysis using 83626-5-RR (same clone as 83626-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83626-5-RR (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83626-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using C1orf83 Recombinant antibody (83626-5-RR)

    IF Staining of U2OS using 83626-5-RR (same clone as 83626-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using C1orf83 antibody (83626-5-RR, Clone: 240678F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83626-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83626-5-RR

    Affinity and Kinetic Characterization of 83626-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83626-5-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

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C1orf83 Recombinant antibody, PBS Only

C1orf83 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83626-5-PBS
Clone No.240678F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83626-5-PBS targets C1orf83 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C1orf83 fusion protein Ag15040 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 83
Calculated molecular weight 208 aa, 23 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC035374
Gene Symbol C1orf83
Gene ID (NCBI) 127428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96MN5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Chromosome 1 open reading frame 83 (C1orf83) is also called TCEANC2, a little-studied transcription elongation factor. The rs10788972 variant of the TCEANC2 gene in the PARK10 region is closely associated with sporadic PD in the American population, while both rs10788972 and rs12046178 variants are not associated with sporadic PD in the Chinese Han population (PMID: 25663231; PMID: 26432391).