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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1orf83 Recombinant monoclonal antibody (83626-3-RR)

    IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using C1orf83 Recombinant monoclonal antibody (83626-3-RR)

    IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C1orf83 Recombinant monoclonal antibody (83626-3-RR)

    IF Staining of HEK-293 using 83626-3-RR (same clone as 83626-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf83 antibody (83626-3-RR, Clone: 240678D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using C1orf83 Recombinant monoclonal antibody (83626-3-RR)

    FC experiment of U2OS using 83626-3-RR (same clone as 83626-3-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug C1orf83 Recombinant antibody (83626-3-RR, Clone:240678D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83626-3-RR

    Affinity and Kinetic Characterization of 83626-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83626-3-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

C1orf83 Recombinant monoclonal antibody, PBS Only

C1orf83 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83626-3-PBS
Clone No.240678D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

240678D10, FLJ32112, FLJ39169, RP4 758J24.3, TCEANC2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83626-3-PBS targets C1orf83 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag15040

Product name: Recombinant human C1orf83 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-208 aa of BC035374

Sequence: MDKFVIRTPRIQNSPQKKDSGGKVYKQATIESLKRVVVVEDIKRWKTMLELPDQTKENLVEALQELKKKIPSREVLKSTRIGHTVNKMRKHSDSEVASLAREVYTEWKTFTEKHSNRPSIEVRSDPKTESLRKNAQKLLSEALELKMDHLLVENIERETFHLCSRLINGPYRRTVRALVFTLKHRAEIRAQVKSGSLPVGTFVQTHKK

相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 83
Calculated molecular weight 208 aa, 23 kDa
GenBank accession numberBC035374
Gene Symbol C1orf83
Gene ID (NCBI) 127428
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96MN5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C1orf83, also known as TCEANC2, and the rs10788972 variant of the TCEANC2 gene in the PARK10 region were identified as being strongly associated with sporadic PD in the US population.

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