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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C1orf77 Polyclonal antibody

C1orf77 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 27478-1-AP

Synonyms

C1orf77, CHTOP, FOP, Friend of PRMT1 protein, pp7704, RP1 178F15.2

Validation Data Gallery

Applications:
  • WB analysis using 27478-1-AP

    WB analysis using 27478-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27478-1-AP (C1orf77 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 27478-1-AP

    IF Staining of A431 using 27478-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf77 antibody (27478-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

27478-1-AP targets C1orf77 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1orf77 fusion protein Ag26580 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 77
Calculated molecular weight 248 aa, 26 kDa
Observed molecular weight26-30 kDa
GenBank accession numberBC002733
Gene symbol C1orf77
Gene ID (NCBI) 26097
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C1orf77 antibody 27478-1-APDownload protocol
IF protocol for C1orf77 antibody 27478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols