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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using C1orf56 Polyclonal antibody (25433-1-AP)

    WB analysis of mouse testis using 25433-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25433-1-AP (C1orf56 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C1orf56 Polyclonal antibody (25433-1-AP)

    IHC staining of human liver cancer using 25433-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25433-1-AP (C1orf56 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C1orf56 Polyclonal antibody (25433-1-AP)

    IHC staining of human liver cancer using 25433-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25433-1-AP (C1orf56 Antibody) at dilution of 1:50 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

C1orf56 Polyclonal antibody

C1orf56 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25433-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

UNQ547/PRO1104, RP11 316M1.9, Protein MENT, Methylated in normal thymocytes protein, MENT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25433-1-AP targets C1orf56 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1orf56 fusion protein Ag13860 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 56
Calculated molecular weight 341 aa, 37 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC002469
Gene Symbol C1orf56
Gene ID (NCBI) 54964
RRIDAB_2880077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BUN1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C1orf56, also named as MENT, is involved in control of cellular proliferation. It is extreme expressed in several cancers especially in neuroblastoma. We detected the endogenous protein 35 kDa in mouse testis.

Protocols

Product Specific Protocols
WB protocol for C1orf56 antibody 25433-1-APDownload protocol
IHC protocol for C1orf56 antibody 25433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Mol Biosci

Identification of Prognostic Biomarkers in Patients With Malignant Rhabdoid Tumor of the Kidney Based on mTORC1 Signaling Pathway-Related Genes.

Authors - Chenghao Zhanghuang
View Article