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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse kidney tissue using C1orf54 Polyclonal antibody (30247-1-AP)

    IHC staining of mouse kidney using 30247-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30247-1-AP (C1orf54 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using C1orf54 Polyclonal antibody (30247-1-AP)

    IHC staining of mouse kidney using 30247-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30247-1-AP (C1orf54 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using C1orf54 Polyclonal antibody (30247-1-AP)

    IF Staining of mouse kidney using 30247-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using C1orf54 antibody (30247-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using C1orf54 Polyclonal antibody (30247-1-AP)

    IF Staining of mouse kidney using 30247-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using C1orf54 antibody (30247-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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C1orf54 Polyclonal antibody

C1orf54 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 30247-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, IF-P, ELISA

Synonyms

C1orf54, FLJ23221

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30247-1-AP targets C1orf54 in IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1orf54 fusion protein Ag32876 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 54
GenBank accession numberBC017761
Gene Symbol C1orf54
Gene ID (NCBI) 79630
RRIDAB_3086279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWF1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C1orf54 is a newly identified protein encoded by an open reading frame on chromosome 1. C1orf54 is highly expressed in healthy mouse kidneys and is only expressed in tubular epithelial cells (TECs), not in glomeruli(PMID: 29999589). C1orf54 promotes renal repair and TEC proliferation through PI3K/AKT signalling, which alleviates kidney damage after ischaemia‐reperfusion injury (IRI) (PMID: 29999589).

Protocols

Product Specific Protocols
IHC protocol for C1orf54 antibody 30247-1-APDownload protocol
IF protocol for C1orf54 antibody 30247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols