Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HT-29 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
27930-1-AP targets C1orf35 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14374 Product name: Recombinant human C1orf35 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-263 aa of BC002843 Sequence: MFGSSRGGVRGGQDQFNWEDVKTDKQRENYLGNSLMAPVGRWQKGRDLTWYAKGRAPCAGPSREEELAAVREAEREALLAALGYKNVKKQPTGLSKEDFAEVCKREGGDPEEKGVDRLLGLGSASGSVGRVAMSREDKEAAKLGLSVFTHHRVESGGPGTSAASARRKPRAEDQTESSCESHRKSKKEKKKKKKRKHKKEKKKKDKEHRRPAEATSSPTSPERPRHHHHDSDSNSPCCKRRKRGHSGDRRSPSRRWHDRGSEA 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 1 open reading frame 35 |
| Calculated molecular weight | 263 aa, 29 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC002843 |
| Gene Symbol | C1orf35 |
| Gene ID (NCBI) | 79169 |
| RRID | AB_2881011 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BU76 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
27930-1-AP antibody detects the 35 kDa phosphorylated protein in SDS-PAGE.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for C1orf35 antibody 27930-1-AP | Download protocol |
| WB protocol for C1orf35 antibody 27930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
