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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using C1orf27 Polyclonal antibody (22844-1-AP)

    WB analysis of mouse brain using 22844-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22844-1-AP (C1orf27 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using C1orf27 Polyclonal antibody (22844-1-AP)

    WB analysis of NIH/3T3 using 22844-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22844-1-AP (C1orf27 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C1orf27 Polyclonal antibody (22844-1-AP)

    IHC staining of human breast cancer using 22844-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22844-1-AP (C1orf27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C1orf27 Polyclonal antibody (22844-1-AP)

    IHC staining of human breast cancer using 22844-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22844-1-AP (C1orf27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22844-1-AP targets C1orf27 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1orf27 fusion protein Ag18802 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 27
Calculated molecular weight 454 aa, 51 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC003397
Gene Symbol C1orf27
Gene ID (NCBI) 54953
RRIDAB_2879176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SWX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C1orf27 antibody 22844-1-APDownload protocol
IHC protocol for C1orf27 antibody 22844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

J Biol Chem

Human C1orf27 protein interacts with α2A-adrenergic receptor and regulates its anterograde transport.

Authors - Xin Xu
  • KD Validated
View Article