Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse cerebellum tissue, SH-SY5Y cells, rat cerebellum tissue, rat cerebellm tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
26342-1-AP targets Draxin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23666 Product name: Recombinant human C1orf187 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 171-349 aa of BC021286 Sequence: QVLDAAMEESSTSLAPTMFFLTTFEAAPATEESLILPVTSLRPQQAQPRSDGEVMPTLDMALFDWTDYEDLKPDGWPSAKKKEKHRGKLSSDGNETSPAEGEPCDHHQDCLPGTCCDLREHLCTPHNRGLNNKCFDDCMCVEGLRCYAKFHRNRRVTRRKGRCVEPETANGDQGSFINV 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 1 open reading frame 187 |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC021286 |
| Gene Symbol | C1orf187 |
| Gene ID (NCBI) | 374946 |
| RRID | AB_2880483 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NBI3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C1orf187, also named Draxin(Dorsal repulsive axon guidance protein), is a repulsive axon guidance cue. It is widely expressed in the developing hippocampus, glioma, and all histological types of the lung cancer cell. Draxin was shown to bind a 22-amino-acid peptide of the EGF domain of Netrin-1, and through Draxin/Netrin-1 interaction, Draxin may act as a secreted Netrin-1 antagonist. C1orf187 can inhibit or repel neurite outgrowth from dorsal spinal cord. It inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway (PMID: 29622850).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Draxin antibody 26342-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas | ||
Chem Biol Interact Transcriptomic and proteomic study of cancer cell lines exposed to actinomycin D and nutlin-3a reveals numerous, novel candidates for p53-regulated genes |