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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using C1S Polyclonal antibody (14554-1-AP)

    WB analysis of human plasma using 14554-1-AP (same clone as 14554-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 14554-1-AP (C1S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using C1S Polyclonal antibody (14554-1-AP)

    WB analysis using 14554-1-AP (same clone as 14554-1-PBS)

    rat plasma lysates were subjected to SDS PAGE followed by western blot with 14554-1-AP (C1S antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14554-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using C1S Polyclonal antibody (14554-1-AP)

    WB analysis of human plasma using 14554-1-AP (same clone as 14554-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 14554-1-AP (C1S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C1S Polyclonal antibody (14554-1-AP)

    IHC staining of human liver cancer using 14554-1-AP (same clone as 14554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using C1S Polyclonal antibody (14554-1-AP)

    IHC staining of human liver cancer using 14554-1-AP (same clone as 14554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C1S Polyclonal antibody (14554-1-AP)

    IHC staining of mouse liver using 14554-1-AP (same clone as 14554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14554-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using C1S Polyclonal antibody (14554-1-AP)

    IHC staining of mouse liver using 14554-1-AP (same clone as 14554-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14554-1-PBS in a different storage buffer formulation.

C1S Polyclonal antibody, PBS Only

C1S Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14554-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14554-1-PBS targets C1S in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5977

Product name: Recombinant human C1S protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 166-688 aa of BC056903

Sequence: DDMKNCGVNCSGDVFTALIGEIASPNYPKPYPENSRCEYQIRLEKGFQVVVTLRREDFDVEAADSAGNCLDSLVFVAGDRQFGPYCGHGFPGPLNIETKSNALDIIFQTDLTGQKKGWKLRYHGDPMPCPKEDTPNSVWEPAKAKYVFRDVVQITCLDGFEVVEGRVGATSFYSTCQSNGKWSNSKLKCQPVDCGIPESIENGKVEDPESTLFGSVIRYTCEEPYYYMENGGGGEYHCAGNGSWVNEVLGPELPKCVPVCGVPREPFEEKQRIIGGSDADIKNFPWQVFFDNPWAGGALINEYWVLTAAHVVEGNREPTMYVGSTSVQTSRLAKSKMLTPEHVFIHPGWKLLEVPEGRTNFDNDIALVRLKDPVKMGPTVSPICLPGTSSDYNLMDGDLGLISGWGRTEKRDRAVRLKAARLPVAPLRKCKEVKVEKPTADAEAYVFTPNMICAGGEKGMDSCKGDSGGAFAVQDPNDKTKFYAAGLVSWGPQCGTYGLYTRVKNYVDWIMKTMQENSTPRED

相同性解析による交差性が予測される生物種
Full Name complement component 1, s subcomponent
Calculated molecular weight 77 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC056903
Gene Symbol C1S
Gene ID (NCBI) 716
RRIDAB_2243610
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP09871
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r activates C1s so that it can, in turn, activate C2 and C4. C1s is a glycoprotein of approximately 88 kDa, which is activated by cleavage into an A chain and a B chain.

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