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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C1RL Polyclonal antibody (24820-1-AP)

    WB analysis using 24820-1-AP (same clone as 24820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24820-1-AP (C1RL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24820-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using C1RL Polyclonal antibody (24820-1-AP)

    WB analysis using 24820-1-AP (same clone as 24820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24820-1-AP (C1RL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24820-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using C1RL Polyclonal antibody (24820-1-AP)

    IF Staining of U2OS using 24820-1-AP (same clone as 24820-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using C1RL antibody (24820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24820-1-PBS in a different storage buffer formulation.

C1RL Polyclonal antibody, PBS Only

C1RL Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24820-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C1r like protein, C1r LP, C1RL1, C1r-like protein, C1r-like serine protease analog protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24820-1-PBS targets C1RL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20324

Product name: Recombinant human C1RL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 41-195 aa of BC156780

Sequence: SVLLAQELPQQLTSPGYPEPYGKGQESSTDIKAPEGFAVRLVFQDFDLEPSQDCAGDSVTISFVGSDPSQFCGQQGSPLGRPPGQREFVSSGRSLRLTFRTQPSSENKTAHLHKGFLALYQTVAVNYSQPISEASRGSEAINAPGDNPAKVQNHC

相同性解析による交差性が予測される生物種
Full Name complement component 1, r subcomponent-like
Calculated molecular weight 487 aa, 53 kDa
Observed molecular weight53 kDa, 70 kDa
GenBank accession numberBC156780
Gene Symbol C1RL
Gene ID (NCBI) 51279
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZP8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

C1r-like serine protease analog protein (CLSPa), also named as CIRL contains a CUB domain and a serine protease domain, possessing characteristic catalytic triad. C1RL is upregulated in glioblastoma (GBM) and has been proved to be a prognostic marker in hepatocellular carcinoma and renal cell cancer (PMID: 32993564). C1RL has a calculated molecular weight of 53 kDa, and this antibody can detect a band at 70 kDa due to possible N-glycosylation (PMID: 15358180).

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